Recombinant Streptavidin (r-SA)
Streptavidin is a homotetrameric protein found in the culture broth of Streptomyces avidinii. Similar to avidin, one mole of streptavidin
Read moreThis winter there will be no Pcr diagnostic detection like before the 90′ due to Filtertip shortage
On account of elevated use of Pcr there’s a worldwide scarcity of 1000ul and 1250ul pcr filter suggestions for dna
Read moreGeneration of an Affinity Column with Sepharose
Take 10 ml Sepharose 2B, and wash several times with water by centrifugation. Do not centrifuge too fast as it
Read moreBiotinylation Protocol for Proteins or Peptides
If using biotin-NHS for protein conjugate, do not use Tris buffer, because it contains nitrogen groups and can quench the
Read moreUsing Bovine Serum Albumin (BSA) as a Blocking Agent
10x Phosphate Buffered SalinePBS (to remove methanol from spotting)80g NaCl (1.37 M to 10x)2g KCl (27mm to 10x)Na2HPO4 (80mm to
Read moreWestern Dot Blot Protocol
TBS20 mM Tris, pH 7.4150 mM NaCl TBS TweenTBST (for washing blots)TBS 2LTween-20 (Brown bottle) + 1mL by Pipetting in
Read moreReducing Proteins or Peptides with Dithiothreitol (DTT)
General Protocol for Reducing cysteines in proteins or peptides Solutions Make diothreitol DTT 1M stock solution in water, it is
Read moreProducing A Flag-Tagged Protein
Make sure that you’re interested in the gene sequence and gene sequences from Bendera tag does not contain a restriction
Read moreHow to Infect Mammalian Cell Cultures with Viruses
Split the cells the day before so the following days the cells are in the desired confluency (40-50% for immunofluorescence
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